Researchers have developed a way to determine the viability of pollen germination in marijuana plants, as well as a simple method of storing cannabis pollen for long periods of time, according to a new study.
“This one is big,” Kovalchuk said. “Our protocol allows nearly indefinite storage,” which is “valuable for maintaining large collection of genetics.”
For marijuana cultivators, the results could prove useful, helping them avoid potentially costly mistakes in the process and ensuring consistency and quality in their crops across growing cycles.
They accomplished that by modifying an existing method of assessing germination viability, using a liquid media instead of a solid medium, which “resulted in better image acquisition and quantification of germination,” according to the study.
The team also discovered that pollen can be more or less viable at different stages of the flowering period. The optimal time to extract pollen seems to be during the mid-flowering stage. At that point, it retained “viability the longest with 22 percent of pollen grains successfully germinating after 21 days” of storage in a low-temperature environment.
The study, which was partly funded by the Canadian government, has “several implications,” co-author Igor Kovalchuk told Marijuana Moment.
Ensuring long-term storage of pollen is important for cultivators because of the central role it plays in generating seeds. Taking pollen from a male plant and rubbing it on the hairs of a female plant—typically about halfway through the flowering cycle—will enable the female plant to produce buds that contains seeds, which along with cloning is one way to propagate and maintain strains.
“In conclusion, we have standardized a simple assay for quickly assessing pollen germination in Cannabis sativa,” the study states. “By using our [modified assay], we have demonstrated the loss of pollen viability over time when stored at 4 degrees Celsius, and suggested an optimal time during flower development for pollen collection to maximize longevity during storage.”
To preserve the pollen, the researchers removed any moisture, added the result to baked whole wheat flour and preserving agents and then froze it in liquid nitrogen. When they removed the mixture from the liquid nitrogen and applied it to flowering female plants, it resulted in successful seed formation in all of the subjects.
Researchers have developed a way to determine the viability of pollen germination in marijuana plants, according to a new study.
January 2, 2017 by Jessica Myers
Open Pollen Sacs
You should bear in mind, though, that every strain will be different during this time frame. If you want to breed your cannabis strain or make your cannabis seeds, you may not be sure of how you will collect the pollen and pollinate the plant effectively. Well, glad you are curious. It is easy. Let’s look at how to begin.
Female Plant Pollination
Storing Your Pollen
You should wait about six weeks before harvesting the seeds because that is how long it will take to see a well developed cannabis seed. Be sure to keep the cannabis plant alive before the seeds drop. You can use your seeds immediately or store them in a dry place until you need them. However, always label the seeds, putting the storage date on the label.
Once the male cannabis plants begin to flower, you can collect pollen. If the female plant is feminizing its seeds, you can do the same. As soon as you see the pollen sacs cracking on the marijuana plant as if they are going to open, you are ready to harvest the pollen. One way that you can tell if your pollen is ready to be harvest is to carefully and gently remove the pollen sacs and allow a week for them to dry. After they are dried, you would then place them all in a re-sealable bag. To know for sure that they are ready, just shake up the re-sealable bag with the pollen and it should spill out easily. It is possible that you might have to cut some of the pollen open.
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